Journal: Nutrients

Article Title: Asiatic Acid Prevents the Deleterious Effects of Valproic Acid on Cognition and Hippocampal Cell Proliferation and Survival

doi: 10.3390/nu8050303

Figure Lengend Snippet: The number of Ki-67 positive cells in the SGZ of the hippocampal dentate gyrus. Ki-67 positive cells were stained in the SGZ of the dentate gyrus (green; ( A )). All nuclei were counterstained with propidium iodide (red; ( B ) and figure merged ( C )). The number of proliferating cells in animals receiving only VPA was significantly lower (* p < 0.05) than vehicle, Asiatic acid and Asiatic acid plus VPA groups, while Asiatic acid and Asiatic acid plus VPA groups were significantly higher when compared to the vehicle group (# p < 0.05). One-way ANOVA with LSD post hoc test was used to compare between all groups ( D ).

Article Snippet: Sections ware fixed in 0.5% paraformaldehyde (pH 7.4) for 3 min and then sections were incubated with primary antibody monoclonal mouse Ki-67 (1:150, Vector Laboratory, Inc., Burlingame, CA, USA) at room temperature for 1 h. Sections were then incubated with secondary antibody Alexa 488 rabbit anti mouse IgG (1:300, Invitrogen, Carlsbad, CA, USA) for 40 min and counter stained with propidium iodide (PI) (1:6000, Sigma Aldrich, Inc., St. Louis, MO, USA) for 30 s and mounted in glycerol.

Techniques: Staining